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endpoint pcr reaction solution  (TaKaRa)


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    TaKaRa endpoint pcr reaction solution
    Agarose gel electrophoresis showing the amplicons <t>of</t> <t>endpoint</t> <t>PCR</t> reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .
    Endpoint Pcr Reaction Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+reaction+solution/bio_rxiv__2025__03__18__643970-152-0-14?v=TaKaRa
    Average 97 stars, based on 265 article reviews
    endpoint pcr reaction solution - by Bioz Stars, 2026-07
    97/100 stars

    Images

    1) Product Images from "Understanding wood decay diseases in western redcedar through development of ITS-sequencing and qPCR assays"

    Article Title: Understanding wood decay diseases in western redcedar through development of ITS-sequencing and qPCR assays

    Journal: bioRxiv

    doi: 10.1101/2025.03.18.643970

    Agarose gel electrophoresis showing the amplicons of endpoint PCR reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .
    Figure Legend Snippet: Agarose gel electrophoresis showing the amplicons of endpoint PCR reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .

    Techniques Used: Agarose Gel Electrophoresis, Amplification, Negative Control

    Standard curve analysis to show sensitivity, repeatability, and efficiency of qPCR assays for detection of gDNA of C. weirii . The qPCR assay was run using SYBR Green Master Mix with the taxon-specific primer. Each DNA dilution was tested in triplicate. (A) Amplification plot showing Ct values of pure C. weirii gDNA with tenfold dilutions ranging from 2 ng to 0.2 pg per PCR reaction. Absolute DNA amounts at five final concentrations (100 pg/µl to 10 fg/µl) with three repeats were tested by the qPCR assay. (B) A standard curve generated by plotting the mean Ct values versus the absolute amounts of C. weirii gDNA. Standard curve displaying a relationship between the log 10 of known amount (pg) of target gDNA and Ct value of qPCR amplicon. A linear relationship between log-transformed DNA amounts in PCR reactions and cycle threshold (Ct) values were detected, with qPCR efficiency E = 0.956.
    Figure Legend Snippet: Standard curve analysis to show sensitivity, repeatability, and efficiency of qPCR assays for detection of gDNA of C. weirii . The qPCR assay was run using SYBR Green Master Mix with the taxon-specific primer. Each DNA dilution was tested in triplicate. (A) Amplification plot showing Ct values of pure C. weirii gDNA with tenfold dilutions ranging from 2 ng to 0.2 pg per PCR reaction. Absolute DNA amounts at five final concentrations (100 pg/µl to 10 fg/µl) with three repeats were tested by the qPCR assay. (B) A standard curve generated by plotting the mean Ct values versus the absolute amounts of C. weirii gDNA. Standard curve displaying a relationship between the log 10 of known amount (pg) of target gDNA and Ct value of qPCR amplicon. A linear relationship between log-transformed DNA amounts in PCR reactions and cycle threshold (Ct) values were detected, with qPCR efficiency E = 0.956.

    Techniques Used: SYBR Green Assay, Amplification, Generated, Transformation Assay



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    Agarose gel electrophoresis showing the amplicons <t>of</t> <t>endpoint</t> <t>PCR</t> reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .
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    Agarose gel electrophoresis showing the amplicons <t>of</t> <t>endpoint</t> <t>PCR</t> reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .
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    Agarose gel electrophoresis showing the amplicons <t>of</t> <t>endpoint</t> <t>PCR</t> reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .
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    Agarose gel electrophoresis showing the amplicons <t>of</t> <t>endpoint</t> <t>PCR</t> reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .
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    Agarose gel electrophoresis showing the amplicons <t>of</t> <t>endpoint</t> <t>PCR</t> reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .
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    Image Search Results


    Agarose gel electrophoresis showing the amplicons of endpoint PCR reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .

    Journal: bioRxiv

    Article Title: Understanding wood decay diseases in western redcedar through development of ITS-sequencing and qPCR assays

    doi: 10.1101/2025.03.18.643970

    Figure Lengend Snippet: Agarose gel electrophoresis showing the amplicons of endpoint PCR reactions. Six genomic regions were amplified using PCR primers designed for C. weirii. Genomic DNA was extracted from different decay fungi as PCR templates. Lane 1: Fermentas GeneRuler 100bp plus DNA ladder, Lane 2: negative control; Lane 3: C. weirii , Lane 4: C. sulphurascens , Lane 5: P. subacida; Lane 6: H. occidentale , Lane 7: A. ostoyae .

    Article Snippet: Endpoint PCR reaction solution contained 5µl Premix Taq™ DNA Polymerase (Ex Taq™ Version 2.0) (Takara Bio Inc., San Jose, USA) master mix, 1µl of each PCR primers (10 mM), and 1 ng of gDNA from each individual fungus for a total volume of 10 µl per reaction.

    Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control

    Standard curve analysis to show sensitivity, repeatability, and efficiency of qPCR assays for detection of gDNA of C. weirii . The qPCR assay was run using SYBR Green Master Mix with the taxon-specific primer. Each DNA dilution was tested in triplicate. (A) Amplification plot showing Ct values of pure C. weirii gDNA with tenfold dilutions ranging from 2 ng to 0.2 pg per PCR reaction. Absolute DNA amounts at five final concentrations (100 pg/µl to 10 fg/µl) with three repeats were tested by the qPCR assay. (B) A standard curve generated by plotting the mean Ct values versus the absolute amounts of C. weirii gDNA. Standard curve displaying a relationship between the log 10 of known amount (pg) of target gDNA and Ct value of qPCR amplicon. A linear relationship between log-transformed DNA amounts in PCR reactions and cycle threshold (Ct) values were detected, with qPCR efficiency E = 0.956.

    Journal: bioRxiv

    Article Title: Understanding wood decay diseases in western redcedar through development of ITS-sequencing and qPCR assays

    doi: 10.1101/2025.03.18.643970

    Figure Lengend Snippet: Standard curve analysis to show sensitivity, repeatability, and efficiency of qPCR assays for detection of gDNA of C. weirii . The qPCR assay was run using SYBR Green Master Mix with the taxon-specific primer. Each DNA dilution was tested in triplicate. (A) Amplification plot showing Ct values of pure C. weirii gDNA with tenfold dilutions ranging from 2 ng to 0.2 pg per PCR reaction. Absolute DNA amounts at five final concentrations (100 pg/µl to 10 fg/µl) with three repeats were tested by the qPCR assay. (B) A standard curve generated by plotting the mean Ct values versus the absolute amounts of C. weirii gDNA. Standard curve displaying a relationship between the log 10 of known amount (pg) of target gDNA and Ct value of qPCR amplicon. A linear relationship between log-transformed DNA amounts in PCR reactions and cycle threshold (Ct) values were detected, with qPCR efficiency E = 0.956.

    Article Snippet: Endpoint PCR reaction solution contained 5µl Premix Taq™ DNA Polymerase (Ex Taq™ Version 2.0) (Takara Bio Inc., San Jose, USA) master mix, 1µl of each PCR primers (10 mM), and 1 ng of gDNA from each individual fungus for a total volume of 10 µl per reaction.

    Techniques: SYBR Green Assay, Amplification, Generated, Transformation Assay